The Effectiveness of Beneficial Microbial Fertilizers in Agriculture
April 2, 2025 | by Aria Thorne

Microbial fertilizers, derived from beneficial bacteria, play a vital role in sustainable agriculture by enhancing soil fertility and reducing the need for chemical fertilizers. Among these, Rhizobium stands out as a key player, particularly for leguminous crops. This article explores the functionality of Rhizobium microbial fertilizers, their types, application methods, and their impact on soil health and crop productivity.
What is Rhizobium?
Rhizobium is a microscopic bacterium invisible to the naked eye without a microscope. Its name originates from Greek: “rhiza” meaning root and “bios” meaning life, reflecting its symbiotic relationship with plant roots. This beneficial bacterium resides in the root nodules of leguminous plants (plants that produce pods, such as peas, beans, and lentils), forming small growths or nodules. Inside these nodules, Rhizobium captures nitrogen directly from the atmosphere, converts it into organic nitrogen compounds, and enriches the soil. This process, known as nitrogen fixation, makes the soil more fertile, reducing the dependency on nitrogen-based chemical fertilizers.
The presence of Rhizobium not only benefits the host plant but also increases the soil’s nitrogen content, making it advantageous for subsequent crops. Rhizobium microbial fertilizers are commercially produced from these bacteria to enhance agricultural productivity sustainably.

Specificity of Rhizobium Strains
Not all Rhizobium bacteria are universally effective across leguminous crops. Different species and strains of Rhizobium are suited to specific crops or groups of crops. For instance, the Rhizobium strain required for soybeans differs from that needed for chickpeas. However, some strains are versatile enough to form nodules in multiple crops within the same group. For example, the same Rhizobium species supports both peas and lentils.
Leguminous crops are categorized into groups based on their compatibility with specific Rhizobium species. Once a farmer cultivates a crop from a particular group, the soil retains sufficient Rhizobium bacteria to support other crops from the same group in future seasons. A notable example is Azorhizobium caulinodans, which forms nodules not only on the roots but also on the stems of plants like sesbania (dhaincha), enhancing nitrogen fixation further.
Key Facts About Rhizobium Microbial Fertilizers
To ensure the effectiveness of Rhizobium fertilizers, certain conditions and standards must be met:
- Carrier Medium: Rhizobium can be stored in mediums like charcoal, organic manure, or lignite. Charcoal is preferred as it can be sterilized, unlike organic materials, reducing contamination risks.
- Viable Cell Count: Each gram of Rhizobium fertilizer should contain at least 100 live bacterial cells.
- Strain Adaptability: The selected strain must be capable of forming nodules under varying climatic conditions.
- Purity of Medium: The carrier medium should be free from other contaminating microbes.
- pH Level: The medium’s pH should range between 6.0 and 7.5 for optimal bacterial survival.
- Effectiveness: Plants treated with Rhizobium should exhibit a 50% higher dry weight compared to untreated plants.
- Packaging: Rhizobium cultures should be stored in polyethylene bags to maintain viability.
- Labeling Requirements: Each fertilizer packet must include:
- Product name
- Target crop
- Manufacturing date
- Expiry date
- Batch number
- Storage instructions (e.g., “Store in a cool, dry place away from direct sunlight and heat”)
- Usage instructions
- Manufacturer’s name and address
Rhizobium Species for Different Crops

Below is a table listing Rhizobium species suited to various leguminous crop groups:
Group Name | Crops | Rhizobium Species |
---|---|---|
Alfalfa Group | Alfalfa, Lucerne, Fenugreek | Ensifer meliloti |
Clover Group | Clover, Berseem | Rhizobium trifolii |
Bean Group | Kidney Bean (Rajma) | Rhizobium phaseoli |
Lupin Group | Lupin | Rhizobium lupini |
Pea Group | Pea, Vetch, Lentil, Lathyrus | Rhizobium leguminosarum |
Soybean Group | Soybean | Bradyrhizobium japonicum |
Cowpea Group | Cowpea, Pigeon Pea, Mung Bean, Chickpea, Peanut | Rhizobium sp. |
Rhizobium Strains for Pulse Crops
Specific strains of Rhizobium are developed for pulse crops to maximize nitrogen fixation. Here’s a table of suitable strains:
Pulse Crop | Rhizobium Strains |
---|---|
Pigeon Pea | CC-1, IHP-195, A-2, A-19, IC-3100 |
Mung Bean | MG-5, KM-1, M-10, GMBS-1 |
Black Gram | BM, BSP-47, PUR-16, PUR-34, CRU-7, DU-106, KU-1, BKR-1-04 |
Chickpea | F-6, F-75, H-45, IC-76 |
Lentil | LB-4, LLR-22, LR-51-97, LRB-1, LRB-2, LWBR-1, L-1-77, L-21-83 |
Pea | P-10-76 |
Lathyrus | K-5 |
How Rhizobium Works
Nitrogen is a critical component of proteins, essential for the growth of all living organisms, including plants. Although the atmosphere contains approximately 77.17% nitrogen, it exists in an inert form that plants cannot directly utilize. Rhizobium bridges this gap by fixing atmospheric nitrogen and converting it into a usable form for plants.
In leguminous plants, Rhizobium resides in root nodules, where it absorbs nitrogen from the air and transforms it into organic compounds. In return, the plant supplies the bacteria with carbohydrates, establishing a mutually beneficial relationship. This process not only sustains the plant but also enriches the soil with residual nitrogen, benefiting subsequent crops.
The efficiency of nitrogen fixation depends on the quality of the nodules. Healthy, reddish nodules indicate robust Rhizobium activity, leading to higher nitrogen fixation rates.
Nitrogen Fixation Rates by Leguminous Crops

The amount of nitrogen fixed in the soil varies by crop. Below is a table showing the nitrogen fixation potential of different leguminous crops:
Crop | Nitrogen Fixed (kg/bigha) | Crop | Nitrogen Fixed (kg/bigha) |
---|---|---|---|
Pea | 7-10 | Pigeon Pea | 23-38 |
Mung Bean/Black Gram | 8-11 | Cowpea | 10-48 |
Chickpea | 12-15 | Soybean | 7-23 |
Lentil | 12-15 | Peanut | 10 |
Benefits for Farmers
Cultivating leguminous crops with Rhizobium reduces the need for synthetic nitrogen fertilizers in subsequent non-leguminous crops like rice or jute. The residual nitrogen left in the soil allows farmers to apply less fertilizer than recommended, cutting costs and promoting eco-friendly farming practices.
Conclusion
Rhizobium microbial fertilizers are a cornerstone of sustainable agriculture, offering a natural solution to enhance soil fertility and crop yield. By understanding the specific strains and application methods, farmers can harness the full potential of these beneficial bacteria. Whether you’re growing pulses, oilseeds, or fodder crops, integrating Rhizobium into your farming practices can lead to healthier soils and a more productive harvest.
For more insights on beneficial microbes in agriculture, check out our previous article “Role of Beneficial Microorganisms in Agriculture”.
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